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Figure 5
Confidence maps benefit from local SNR adjustment based on local resolution. (a) Locally filtered β-galactosidase (EMD-2984) cryo-EM map (gray) displayed at a 4.5σ threshold (left) and (b) confidence map (blue) including signal-to-noise adjustment based on local resolution at a 1% FDR threshold (right) in side view and cross-section. High-resolution features such as Glu304–Glu398 and holes in the aromatic rings of Trp585 in the 3.5/4.5σ-thresholded cryo-EM map (a) in comparison with the 1% FDR confidence map (b). (c) Comparison of density features from peripheral loop regions not covered by density in the locally filtered cryo-EM map (left) compared with the 1% FDR confidence map that shows densities for the respective loops.

IUCrJ
Volume 6| Part 1| January 2019| Pages 18-33
ISSN: 2052-2525
https://doi.org/10.1107/S2052252518014434